| The Etiologic Roles and Carcinogenic Mechanisms of Human Papilloma Virus in Head and Neck Squamous Cell Carcinoma |
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Dong Hyun Shin;Sei Young Lee;Bon Seok Koo;Se-Heon Kim |
| 두경부 편평세포암종의 발암 원인으로 인간유두종 바이러스(Human Papilloma Virus)의 역할 및 이와 관련된 발암 기전에 관한 연구 |
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신동현;이세영;구본석;김세헌 |
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| Abstract |
Background ：The most frequently reported risk factors for head and neck suamous cell carcinoma are smo-king and alcohol. But in a recent overview, human papilloma virus(HPV) infection was revealed the important carcinogenic factor in oropharyngeal cancer. We aimed to clarify whether HPV directly effects on the oncoge-nesis and biologic behavior of hean and neck squamous cell carcinoma by comparison with infection preva-lence, and physical status of virus.
Material and Method ：We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oli-gonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner.
The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5’-exonu-clease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene.
Results
：We observed a significant difference in HPV prevalence between tonsillar cancer group and con-trol group(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%) and integrated(94.1%) state. In terms of oral tongue cancer, we demonstrate that 30.5% has integrated HPV-16 in cancer tissue. But Glottic cancer only 1% is related to HPV-16 integration.
Conclusion
：This study revealed significant relationship of HPV prevalence with oropharyngeal and oral tongue squamous cell carcinoma. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to the carcinogenesis. |
| Key Words:
Human papillomavirus, Integration, Head and neck squamous cell carcinoma |
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