Tre Effect of UW Solution for Protection of Ischemic Injury in Free Myocutaneous Flaps of the Rabbit |
Woo Suk Suh;Woo Heung Kwun;Sang Woon Kim;Su Jung Lee;Koing Bo Kwun |
가토 근피판에서 허혈성 손상 방지를 위한 UW 관류제 사용의 효과 |
서우석;권우형;김상운;이수정;권굉보 |
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Abstract |
The benficial effects for perfusion in the preservation of free flaps have been controversial in the clinical and experimental field until now.
This study was undertaken to observe the effect of UW solution. a recently developed. high molecular weight. organ perfusion solution. for protection of ischemic injury in normothermic free myocutaneous flaps.
Forty rabbits were used in this sutdy. A 1x2x1cm sized gastrocnemius myocutaneous flap based on the feeding vessel from common femoral artery was made. The author set up the ischemic time for 12 hours in these flaps. The flap was washed out with normal saline(control grop, n=10), urokinase(comparative group I, n=10), UW solution before ischemic time(comparative group II, n=10) and UW solution before ischemic time and pentoxifylline before reperfusion(comparative group III, n=10). Afterthen, reperfusion was made for 12 hours.
After this procedure, we checked the degree of ischemia and necrosis of myocutaneous flap by gross finding, electrical stimulation test of muscle, triphenyltetrazolium chloride staining and wet/dry weight ratio.
The degree of necrosis of comparative group II and III were lesser than control and urokinase group in gross finding(p 1.76±1.01) and urokinase( 2.36±1.02 ) group however the muscular power of comparative group II( 3.54±0.93) and III( 3.49±1.37gm/mm²) demonstrated significantly higher than control group(p 4.55±0.29) and III( 3.75±0.48) were scored significantly lesser than control and urokinase group (p<0.05).
These results suggest that perfusion washout with UW solution improves the viability of normothermic free myocutaneous flap by inhibition of cellular swelling. |
Key Words:
UW solution, Myocutaneous flap |
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