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Korean Journal of Head & Neck Oncology 2008;24(1):33-42.
Published online May 30, 2008.
Cytogenetic Analysis in Korean Head and Neck Cancer Cell Lines:Comparative Genomic Hybridization(CGH) and Array-CGH
You Ree Shin;Soo-Yeun Park;Dong Wook Lee;Han Su Kim;Young Min Go;Hyun Joo Park;Sung Min Choung
두경부 편평상피세포암 세포주의 염색체 이상 분석:비교유전체보합법과 Array 비교유전체보합법
신유리;박수연;이동욱;김한수;고영민;박현주;정성민
Abstract
Head and neck squamous cell carcinoma(HNSCC) is notorious for its poor outcome and increasing incidence. But, the studies of cytogenetic analysis in HNSCC are relatively rare, because of difficulties in culturing solid tumor cells and complexity in chromosomal DNA abberations associated with the lesions. The purpose of this study is to evaluate the location of chromosomal aberrations in Korean HNSCC cell lines (SNU-1041, 1066, and 1076) with comparative genomic hybridization(CGH) and array based CGH(array-CGH). Chromosomal gains of 3q23-q27, 5p13-p15.3, 7p21-pter, 8q11.2-q12, 8q21.1-qter, 9q22-q34, 16q22-q24, and 20q11.2-qter, as well as chromosomal losses on 3p10-p14 were found in all 3 SNU cell lines. Losses on 3p15-p23, 4q22-q27, 4q31.3-qter, 6q14-q15, 7q31-q34, 8p12-pter, 18q21-q23, and 21q11.2-q12 were observed in 2 of 3 cell lines. In array-CGH, many genes were altered including gains of PIK3CA, MYC, EVI1, MAD1L1 genes and losses of SERPIN genes. These aberrations of gene and chromosome coincide with other results of study, generally. These data about the patterns of chromosomal aberrations could be a basic step for understanding more de-tailed genetic events in the carcinogenesis and also provide information for diagosis and treatment in HNSCC.
Key Words: Genomic hybridization, Head and neck cancer, Squamous cell carcinoma, Cytogenetic analysis, array 기반 비교 유전체 보합법


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