| Induction of Apoptosis in FRTL-5 Thyroid Cells by Okadaic Acid |
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Ji Hyoung Cho;Ki Yong Chung;Jong Wook Park |
| Okadaic Acid에 의한 FRTL-5 갑상선 세포주의 Apoptosis 유도 |
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조지형;정기용;박종욱 |
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| Abstract |
Objectve : Okadaic acid is a specific inhibitor of serine/threonine protein phosphatase 1 and 2A. In order to know the mechanism of apoptosis induced by okadaic acid, we treated FRTL-5 thyroid cells with okadaic acid and measured the changes of important proteins that are involved in apoptosis.
Materials and Methods: We measured caspase 3 activity, PLC−γ1 degradation, the expression of XIAP, cIAP1, cIAP2, and cytochrome c release in okadaic acid-treated FRTL-5 thyroid cells.
Results
Okadaic acid-induced caspase 3 activation and PLC−γ1 degradation and apoptosis were dose-dependent with a maximal effect at a concentration of 80 nmol and time-dependent with a maximal effect at 24 hours after treatment. The elevated caspase 3 activity in okadaic acid treated FRTL-5 thyroid cells are correlated with down-regulation of XIAP and cIAP1, but not cIAP2. General and potent inhibitor of caspases, z-VAD-fmk. abolished okadaic acid-induced caspase 3 activity and PLC−γ1 degradation. The release of cytochrome c in okadaic acid-induced FRTL-5 thyroid cells was dose-dependent with a maximal effect at a concentration of 80 nmol.
Conclusions
These findings suggest that mechanism of okadaic acid-induced apoptosis is associated with cytochrome c release and increase of caspase 3 activation in FRTL-5 thyroid cells. |
| Key Words:
Thyroid carcinoma, Okadaic acid, Apoptosis, Caspase 3 |
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